Digital Polymerase Chain
Reaction in an Array of Femtoliter
Polydimethylsiloxane Microreactors
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Abstract
We developed a simple, compact microfluidic device to
perform high
dynamic-range digital polymerase chain reaction (dPCR) in an array
of isolated 36-femtoliter microreactors. The density of the microreactors
exceeded 20 000/mm<sup>2</sup>. This device, made from polydimethylsiloxane
(PDMS), allows the samples to be loaded into all microreactors simultaneously.
The microreactors are completely sealed through the deformation of
a PDMS membrane. The small volume of the microreactors ensures a compact
device with high reaction efficiency and low reagent and sample consumption.
Future potential applications of this platform include multicolor
dPCR and massively parallel dPCR for next generation sequencing library
preparation