Giant Vesicles Containing
Superparamagnetic Iron Oxide
as Biodegradable Cell-Tracking MRI Probes
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Abstract
A major breakthrough in <i>in vivo</i> cellular
imaging
has been the clinical/preclinical use of magnetic resonance imaging
(MRI) with contrast agent. Superparamagnetic iron oxide (SPIO) is
a promising candidate for the development of smart MRI probes for
cell-tracking. In the present study, we describe biodegradable probes
made of giant vesicles (GVs; closed lipid membranes with diameters
>1 μm) that encapsulate SPIO for use as an MRI contrast agent.
These SPIO-containing GVs (SPIO-GVs) exhibited excellent contrast
enhancement in the single cell of medaka fish (<i>Oryzias latipes</i>) embryos immediately after their microinjection, and this enhancement
disappeared when the GV membranes were destroyed. Our results demonstrate
that SPIO-GVs are useful MRI probes for single cell-tracking that
have minimum cytotoxicity and will greatly improve clinical/preclinical <i>in vivo</i> cellular imaging techniques