Synthesis and in Vitro Evaluation of a Biotinylated
Dextran-Derived Probe for Molecular Imaging
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Abstract
Herein we report the design, synthesis, and in vitro evaluation
of a gadolinium-containing biotinylated dextran-derived molecular
imaging probe as a prospective neuroanatomical tracer by means of
magnetic resonance imaging (MRI). The probe was effectively taken
up by cultured differentiated murine neuroblastoma cells and significantly
enhanced the contrast in <i>T</i><sub>1</sub>- and <i>T</i><sub>2</sub>-weighted MR images of labeled cells under
physiological conditions. A significant longitudinal relaxation rate
enhancement in the presence of avidin was observed allowing the verification
of the results in the end of noninvasive longitudinal MRI connectivity
studies by post-mortem histology. The in vitro results indicate that
the probe has the potential to be used in vivo to identify the organization
of global neuronal networks in the brain with MRI