Peroxiredoxins 3 and 4
Are Overexpressed in Prostate Cancer Tissue and Affect the Proliferation
of Prostate Cancer Cells in Vitro
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Abstract
The present study aimed to investigate the proteome profiling
of surgically treated prostate cancers. Hereto, 2D-DIGE and mass spectrometry
were performed for protein identification, and data validation for
peroxiredoxin 3 and 4 (PRDX3 and PRDX4) was accomplished by reverse
phase protein arrays (RPPA). The Formal Concept Analysis (FCA) method
was applied to assess whether the TMPRSS2-ERG gene fusion could influence
the degree of overexpression of PRDX3 and PRDX4 in prostate cancer.
Lastly, we performed an <i>in vitro</i> functional characterization
of both PRDX3 and PRDX4 using the classical human prostate cancer
cell lines DU145 and LNCaP. Reverse phase protein arrays verified
that the overexpression of both PRDX3 and PRDX4 in tumor samples is
negatively correlated with the presence of the TMPRSS2-ERG gene fusion.
Functional characterization of PRDX3 and PRDX4 activity in PCa cell
lines suggests a role of these members of the peroxiredoxin family
in the pathophysiology of this tumor entity