Reversible Regulation
of Protein Binding Affinity
by a DNA Machine
- Publication date
- Publisher
Abstract
We report a DNA machine that can reversibly regulate
target binding
affinity on the basis of distance-dependent bivalent binding. It is
a tweezer-like DNA machine that can tune the spatial distance between
two ligands to construct or destroy the bivalent binding. The DNA
machine can strongly bind to the target protein when the ligands are
placed at an appropriate distance but releases the target when the
bivalent binding is disrupted by enlargement of the distance between
the ligands. This “capture–release” cycle could
be repeatedly driven by single-stranded DNA without changing the ligands
and target protein