Glial Csk regulates Fasciclin II localization at the NMJ.

Abstract

<p><b>(A-E)</b> Immunostaining of anti-FasII (green), anti-Dlg (red), and anti-HRP (blue) at NMJs with the following genotypes: <b>(A-A”)</b> wild type, <b>(B-B”)</b> <i>Csk</i>^{<i>c04256</i>}, <b>(C-C”)</b> <i>Csk</i>^{<i>c04256</i>}<i>/Csk</i>^<i>j1D8</i>, <b>(D-D”)</b> <i>Csk-RNAi</i> expressed in the whole animal (<i>Tubulin-Gal4</i>), and <b>(E-E”)</b> <i>Csk-RNAi</i> expressed only in glia (<i>Nrv2-Gal4</i>). Extra-synaptic FasII was defined as FasII signal found outside the Dlg-stained region. Areas with high levels of extra-synaptic FasII are indicated with white arrows. Scale bar = 10 μm for A and 5 μm for A”. <b>(F)</b> Relative levels of extra-synaptic FasII debris (extra-synaptic FasII staining area/total FasII staining area) present at the synapse. Values are represented as a percent of wild type to allow for appropriate comparison between multiple immunostaining experiments. For details on quantification of extra-synaptic FasII levels, see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005886#sec015" target="_blank">Methods</a>. * <i>p</i> < 0.05, ** <i>p</i> < 0.01, *** <i>p</i> < 0.001 by Student’s T-test compared to wild type.</p