Expression of a Fasciclin II isoform is lowered during synaptic homeostasis and regulated by <i>Csk</i>.

Abstract

<p><b>(A-E)</b> Representative images of FasII immunostaining at NMJs that are <b>(A)</b> wild type, <b>(B)</b> <i>Csk</i>^{<i>c04256</i>}<i>/Csk</i>^<i>j1D8</i>, <b>(C)</b> expressing <i>Csk-RNAi</i> in muscle and neurons, <b>(D)</b> expressing <i>Csk-RNAi</i> in only muscle, <i>and</i> <b>(E)</b> expressing <i>Csk-RNAi</i> in only neurons. <b>(F)</b> Average values for synaptic FasII fluorescence intensity normalized to synapse area and normalized to wild type. <b>(G)</b> Western blot of FasII (DSHB 1D4 antibody) in protein extracts from whole third instar larvae. <b>(H, I)</b> Relative quantification of (H) total FasII intensity and (I) the intensity of the lowest molecular weight FasII band (the ‘third band’). Quantification in H and I is shown as a fold change relative to wild type. Values on/above bars indicate the number of biological replicates for each genotype. * <i>p</i> < 0.05, ** <i>p</i> < 0.01, *** <i>p</i> < 0.001, ns—not significant (<i>p</i> > 0.2) by Student’s T-test compared to wild type.</p