<p>(A) Total promastigote cell extracts were separated in the first dimension with a pH gradient of 5–8 (7-cm strips) followed by 12% SDS-PAGE. The separated proteins were electroblotted onto a nitrocellulose membrane and probed with pools of sera from (B) asymptomatic (n = 9; 1:500) and (C) symptomatic dogs (n = 5; 1:1,000). Protein spots recognized by AD sera only are numbered.</p
