<p><i>rrf-3(pk1426)</i> mutant hermaphrodites were cultured at 20°C with bacteria containing the control RNAi plasmid (L4440, blue) or the <i>acn-1</i> RNAi plasmid (red) starting at the embryonic stage. (A) Body movement was assessed by counting body bends with a dissecting microscope. (B) Images show bacterial lawns. Day 15 adults were cultured on fresh, smooth lawns for two hours–lines are tracks caused by moving worms. Animals treated with <i>acn-1</i> RNAi generated more tracks than control animals, and the tracks are suggestive of more coordinated sinusoidal movement compared to control animals. (C) Pharyngeal pumping was assessed by counting pumps with a dissecting microscope. n.s., <i>P</i> > 0.05; *, <i>P</i> < 0.05; **, <i>P</i> < 0.005; ***, <i>P</i> < 0.0001.</p
