<p>(A) Heterozygous <i>stxbp1a</i><sup><i>s3000/+</i></sup> mutant larvae (5 dpf) are morphologically indistinguishable from wild-type siblings. Homozygous <i>stxbp1a</i><sup><i>s3000/s3000</i></sup> mutant larvae are immobile and fail to hatch out of the chorion. Scale bar = 500 μm. (B) Homozygous <i>stxbp1a</i><sup><i>s3000/s3000</i></sup> mutant larvae (n = 10) removed from their chorions are not significantly different in length from their siblings (n = 30; p = 0.0592, two-tailed t-test). (C-D) At 5 dpf, the dorsal surface of homozygous <i>stxbp1a</i><sup><i>s3000/s3000</i></sup> mutant larvae that were removed from their chorions at 2 dpf (D) show dispersed melanin and foreshortened craniofacial structure compared to siblings (C). Scale bar = 300 μm.</p
