<p>(A) Mean trajectory angles of <i>eat-4(ky5)</i>, <i>ceh-36(ky646)</i> and <i>nsy-5/inx-19</i> (AWC<sup>OFF/OFF</sup>) mutant animals are significantly different from wild type at 1.5 and 3 V/cm (<i>p</i> ≤ 0.0001). <i>nsy-1</i> (AWC<sup>ON/ON</sup>) mutant animals’ mean trajectory angle are slightly wider than that of wild-type animals at 1.5 and 3 V/cm (Fig 4A; <i>p</i> ≥ 0.0035). <i>eat-4(</i>+<i>)</i> rescue in AWC neurons (AWC::<i>eat-4</i>) results in a slight lowering of mean trajectory angle compared to <i>eat-4</i> mutant animals at 3 V/cm (<i>p</i> ≥ 0.0057). (B) Mean speed of different strains. <i>eat-4</i> and <i>ceh-36</i> mutant animals fail to increase speeds upon application of an electric field stimuli (1.5-6V/cm) when compared to wild type (<i>p</i> ≤ 0.007 and <i>p</i> ≤ 0.0067,respectively). <i>nsy-5/inx-19</i> (AWC<sup>OFF/OFF</sup>) animals do not differ statistically from non-stimulus speeds (0 V/cm) at 1.5V/cm and 3V/cm (<i>p</i> ≥ 0.0702). <i>nsy-1</i> (AWC<sup>ON/ON</sup>) animals have a significant increase in speed at all field strengths compared to non-stimulus (0 V/cm) speeds (<i>p</i> ≤ 0.0005). <i>eat-4(</i>+<i>)</i> rescue in AWC neurons (AWC::<i>eat-4</i>) results in increased speeds at all field strengths (1.5–6 V/cm; <i>p</i> ≤ 0.007). Error bars represent SEM; number of individual animal tracks per genotype (<i>N</i>) ≥ 154 per calculated average. Data points for different strains were staggered for better visualization. <i>P</i>-values were determined from post-hoc pairwise comparisons of estimates obtained via general linear mixed-models. Significance was assessed using a false discovery rate of 0.05.</p
