Transcript expression analysis of <i>phist</i> and <i>resa-like</i> genes during <i>P</i>. <i>falciparum</i> intraerythrocytic stages.

Abstract

<p>Gene-specific transcript levels for select <i>phist</i> genes <b>A</b>) and for the <i>resa-like</i> genes <b>B</b>) were analyzed by real time PCR using cDNA prepared from intraerythrocytic synchronized asexual and gametocyte <i>P</i>. <i>falciparum</i> cultures. Note that <i>resa</i> (<i>PFA0110w</i>) and <i>resa2</i> (<i>PF11_0509</i>) are highly expressed relative to the other <i>resa-like</i> genes. Transcript expression was normalized to the expression of the control gene <i>arginyl tRNA synthetase</i> (<i>PFL0900c</i>). Hours post-synchronization indicate time in hours after adding purified schizonts to fresh blood culture. In the schematic depiction of <i>phist</i> and <i>resa-like</i> genes, yellow represents the signal sequence, and red represents the PEXEL/HT motif. The life cycle stage percentage in the population for each time point is shown in <b>C</b>). The letter “G” indicates mature gametocytes.</p

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