<p>Periodic measurements of 4-h fasted blood glucose in (<b>a</b>) cohort A (n = 12–18, most time points) and (<b>b</b>) cohort B (n = 8–10, most time points) is shown, in addition to weekly body mass in (<b>c</b>) cohort A (n = 12–18, most time points) and (<b>d</b>) cohort B (n = 8–10, most time points). (<b>e</b>) Body mass was also tracked in a subset of pre-weaned male pups from cohort B (n = 12–22). (<b>f</b>) Litter sizes were compared between cohorts, with scatter points indicating the number of pups per individual litter (n = 12–16). (<b>g</b>) 4-h fasted corticosterone levels, measured in plasma collected during early afternoon from 27 week-old mice, were assessed between cohorts. Data are means ± SEM. (<b>h</b>) The relationship between corticosterone and insulin levels across cohorts in 27 week-old HFD-fed males (cohort A: closed points, n = 10; cohort B: open points, n = 9-10), with r<sup>2</sup> = 0.38 and <i>p</i> < 0.01 for <i>Ins1</i><sup>-/-</sup>:<i>Ins2</i><sup>+/-</sup> mice. (<b>i</b>) The relationship between body mass and 4-h fasted insulin levels at one year of age, r<sup>2</sup> = 0.55 and <i>p</i> < 0.0001 (cohort A: closed points, n = 9–12; cohort B: open points, n = 6–7). Dark blue and red represent CD- and HFD-fed <i>Ins1</i><sup>-/-</sup>:<i>Ins2</i><sup>+/+</sup> male mice, respectively; pale blue and orange represent CD- and HFD-fed <i>Ins1</i><sup>-/-</sup>:<i>Ins2</i><sup>+/-</sup> male mice, respectively. <i>p</i> ≤ 0.05 denoted by * for CD vs HFD, # for <i>Ins1</i><sup>-/-</sup>:<i>Ins2</i><sup>+/+</sup> vs <i>Ins1</i><sup>-/-</sup>:<i>Ins2</i><sup>+/-</sup>.</p
