<p>(A) <i>Bma-aldolase-1</i> and <i>Bma-aldolase-2</i> are overexpressed in adult female worms as compared to microfilaria (Mf). Relative gene expression between females and Mf was calculated using the ΔΔCt method. The median value of the Mf group was set to 100% and the increase in expression in the female worms was calculated as a percentage of the control. (B) Western blot analysis shows the expression of BMA-ALDO-1 and BMA-ALDO-2 proteins in Mf (lane 1), in 6-day doxycycline-treated Mf (lane 2), in adult females (lane 3), and in 6-day doxycycline-treated adult female worms (lane 4). Reduction of <i>Bma-aldolase-2</i> (C) and <i>Bma-aldolase-1</i> (D) expression in doxycycline (3- and 6-day, grey bars) treated female worms as compared to control untreated worm samples (transparent bars). The median value of the control group was set to 100% and the reduction in expression in the treated groups was calculated as a percentage of the control. *: <i>p</i> < 0.001, NS: not significant.</p
