<p><b>(A) Kaplan-Meier survival curve of worm populations exposed to zinc or TPEN as larvae</b>. Wildtype worms were exposed to 500μM ZnSO<sub>4</sub> or 200μM TPEN at the L3 stage. Mean and maximum population lifespan were reduced with zinc and increased with TPEN treatment (p < 0.0001, log rank test) <b>(B) Kaplan-Meier survival curve of worm populations exposed to zinc or TPEN as adults</b>. Wildtype worms were exposed to 500μM ZnSO<sub>4</sub> or 200μM TPEN at day 5 of adulthood. Thl.ere was no significant difference in mean and maximum population lifespan due to zinc or TPEN treatment. <b>(C) Changes in total zinc content in worms exposed to zinc or TPEN as larvae</b>. Wildtype worms were exposed to 500μM ZnSO<sub>4</sub> or 200μM TPEN at the L3 stage and collected at one-day old adult animals for analysis of total zinc content. Worms supplemented with zinc had a ~2-fold increase in total zinc content (***, p<0.0001, t-test), while worms treated with TPEN demonstrated a ~2-fold decrease in total zinc content (**, p<0.001, t-test). Data shown are the mean ± SD of 3 experimental replicates.</p
