Reduced solubility due to ZnCl₂ and CuCl₂ is reversed by EDTA and SDS application.

Abstract

<p>Pooled brain tissues derived from C57BL wild-type mice (A) and bovine (B) homogenized in TBS and 2% N-octyl-β-D-glucopyranoside were supplemented with ZnCl₂ (1 mM) and CuCl₂ (1 mM) followed by incubation in the absence (0) or presence of EDTA in concentrations indicated or 1% SDS. Following centrifugation, proteins were separated into fractions of high solubility in the supernatant and low solubility in the pellet. PrP^C signals were detected using mab SAF34 and visualized by chemiluminescence substrate development.</p