Comprehensive
Proteomic Study of the Antiproliferative
Activity of a Polyphenol-Enriched Rosemary Extract on Colon Cancer
Cells Using Nanoliquid Chromatography–Orbitrap MS/MS
In this work, a proteomics strategy
based on nanoliquid chromatography–tandem
mass spectrometry (nano-LC–MS/MS) using an Orbitrap high-resolution
mass spectrometer together with stable isotope dimethyl labeling (DML)
is applied to quantitatively examine relative changes in the protein
fraction of HT-29 human colon cancer cells treated with different
concentrations of a polyphenol-enriched rosemary extract over the
time. The major objective of this study was to gain insights into
the antiproliferative mechanisms induced by rosemary polyphenols.
Using this methodology, 1909 and 698 proteins were identified and
quantified in cell extracts. The polyphenol-enriched rosemary extract
treatment changed the expression of several proteins in a time- and
concentration-dependent manner. Most of the altered proteins are implicated
in the activation of Nrf2 transcription factor and the unfolded protein
response. In conclusion, rosemary polyphenols induced proteomic changes
that were related to the attenuation of aggresome formation and activation
of autophagy to alleviate cellular stress