<p>To demonstrate the dynamic changes of somatic hypermutation level (SHM) over time, the mean and 95% confidence interval of SHM was calculated for each time point of the three lineages. Stages from the same chain of a lineage were connected to reveal longitudinal trends. The plots of CDR regions were colored magenta while plots of FWRs were colored blue. (A) Longitudinal somatic hypermutation levels of VRC26 heavy (left) and light (right) chains. The measured SHM against UCA reached substitution saturation around 159 wpi. (B) Longitudinal somatic hypermutation levels of CH103 heavy (left) and light (right) chains. The SHM level from light chain UCA reached substitution saturation around 92 wpi, but the SHM level from heavy chain UCA appears to not reach substitution saturation. (C) Longitudinal somatic hypermutation levels of VRC01 clade 03+06 heavy (left) and light (right) chains. (D) Longitudinal somatic hypermutation levels of VRC01 clade 08 heavy (left) and light (right) chains. (E) Longitudinal somatic hypermutation levels of VRC01 clade H3. (F) Longitudinal somatic hypermutation levels of VRC01 clade L3. The measured SHM from the MRCAs of each clade reached substitution saturation around 2006.</p
