<p>(A-B) Adenosine-mediated TCRP (A) and PMA-mediated TCRP (B) for functional screening of various Radix <i>P</i>. <i>alba</i> components. For details about the five various components, see the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0155930#sec002" target="_blank">Materials and Methods</a>. (C) Immunoblotting analysis of the phosphorylation of Akt and Erk1/2 in RBL-2H3 cells treated with different Radix <i>P</i>. <i>alba</i> components. (D) Effects of the five fractions on [Ca<sup>2+</sup>]<sub>i</sub> mobilization. RBL-2H3 cells were treated with PE, EA, EtOH, and H<sub>2</sub>O extractions, respectively, for 1 h and incubated with 50 μL of 5 mM Fluo-4/AM for 30 min at 37°C after washing three times with HBSS. The cells were monitored in real-time by spectra Max M5 pre- and post-stimulation with PKC. The [Ca<sup>2+</sup>]<sub>i</sub> mobilization was statistically analyzed by comparing the peak fluorescence intensity value which were normalized to the control values. * P < 0.05, ** P < 0.01 vs. control.</p
