Impaired Ca2+ mobilization in Lyn-/- BMMCs is independent of the stimulus concentration. Wt and Lyn-/- BMMCs were preloaded and starved overnight with 0.15 μg/ml IgE. Ca2+ mobilization was measured for 4 min by flow cytometry using the Ca2+-sensitive fluorescent dyes fluo-3 (Ca2+ b) and fura red (Ca2+ ub). Steady-state fluorescence was assessed for 1 min before BMMCs were stimulated with the indicated Ag concentrations. The arrow marks the time point of stimulus addition. Comparable results were obtained with cells from different BMMC cultures (n = 3). (PDF 289 kb
