Additional file 2: Figure S2. of Heat-mediated reduction of apoptosis in UVB-damaged keratinocytes in vitro and in human skin ex vivo

Abstract

(a) Immunohistochemical staining of SIRT1-p (green), total SIRT1 (red) and DAPI (nucleus, blue) in skin samples or primary keratinocytes that were either untreated, or exposed to heat, UVB or UVB plus heat. Broken lines denote the epidermal/dermal border. Scale bar (white line) =100 μm. Inset images are enlarged view of SIRT1/SIRT1-p positive cells, which are also indicated by red arrows. (b) Bar graphs of mean ± SD percent keratinocytes carrying phosphorylated and normal SIRT1 protein in ex vivo skin. (JPG 367 kb