T helper cell inducing conditions have an effect on the intracellular [Zn2+] of CD4+ T cells during primary activation and differentiation. Splenocytes from MT knockout (MT-/-) (n = 3–4) or wildtype controls (MT+/+) (n = 3–4) were stimulated with anti-CD3 and anti-CD28 for 6 days in the presence of no added cytokines (Th0), IL-12 + IL-2 + anti-IL-4 (Th1), or IL-27 (Tr1) to promote differentiation and expansion of T helper cell populations. Intracellular [Zn2+] in the CD4+ T cell population was measured for each inducing condition and MT genotype at +6 days post stimulation. (PDF 90 kb
