<p>(A) Relative expression of several endocrine marker genes was analyzed by QRT-PCR in the pancreas from 4 weeks old WT and Glis3KO2 mice (n ≥ 4). (B, C) Pancreata from 4 weeks old WT and Glis3KO2 mice were examined by DAPI and FITC-DBA (B) or with antibodies against Ppy and insulin (C). (D-E) Exogenous expression of Glis3 induced Ppy mRNA expression in αTC1-9 and HPDE cells. Cells were infected with pLVX-mCherry, pLVX-mCherry-Glis3, -Pdx1, -MafA, or -NeuroD1 lentivirus and expression of Ppy mRNA was analyzed by QRT-PCR. Data present mean ± SEM. * p <0.05. (F) Schematic of the spatiotemporal pattern of expression of Glis3 during pancreas development. DOI <a href="http://dx.doi.org/10.6084/m9.figshare.3189178" target="_blank">10.6084/m9.figshare.3189178</a>.</p
