Glis3 protein was not detectable in E12.5 pancreata, but was selectively expressed at E13.5 of pancreas development.

Abstract

<p>E12.5 pancreata from Glis3^GFP/GFP embryo were double-stained with antibodies against GFP (A-B) and Pdx1 (A) or Sox9 (B). E13.5 Glis3^GFP/GFP pancreata were double-stained with antibodies against GFP (C-G) and Pdx1 (C), Ngn3 (D), Sox9 (E), or Ptf1a (F). Boxed areas were enlarged and shown in the panels on the right. DAPI stained and merged images are indicated. Arrows indicate Glis3⁺Pdx1⁺ (C), Glis3⁺Ngn3⁺ (D) or Glis3⁺Sox9⁺ (E) cells; arrow heads indicate Ptf1a single positive cells (F). Triple staining of E13.5 Glis3^GFP/GFP pancreata with anti-GFP, anti-Nkx6.1, and Sox9 (G). Arrows indicate triple stained cells (G, inset). Dashed circles indicate tip domains; dashed lines indicate trunk domains. Schematic views (shown on the right) of the primary and secondary transition of pancreas development indicating formation of tubular structures of multipotent progenitor cells (MPC) (E12.5) and the compartmentalization and establishment of multipotent progenitors and (pre)acinar cells (A) at the tip domain and the appearance of bipotent progenitors (BP), endocrine progenitors (EP), and preductal cells (D) in the trunk domain (E13.5), respectively. DOI <a href="http://dx.doi.org/10.6084/m9.figshare.3189169" target="_blank">10.6084/m9.figshare.3189169</a>.</p