TTX-sensitive spikes in spiking neurons from mature tissue. A: Representative current-clamp step-protocol traces from a spiking neuron under pre (Control) and post-tetrodotoxin (sodium channel blocker) application (TTX). The step protocol consisted of an initial –200 pA step followed by eight 50 pA steps (750 ms in duration) from an initial V m of –70 mV. B: Summarized data (n = 5) on the effect of TTX (1–0.1 μM) on the number of spikes per second (normalized) under control and TTX conditions in spiking neurons from tissue slices around 1 month old (t (4) = –29.7; p < 0.0001). (PDF 200 kb
