<p>(A) Wild-type E18.5 ovarian chromatin pulled down by antibodies against TAF4b or IgG and then qPCR-amplified using primers against the proximal promoters of <i>Stra8</i>, <i>Dazl</i>, <i>Figlα</i>, <i>Nobox</i> and a non-genic region upstream of <i>Nobox</i>. qPCR demonstrates enriched recovery of these proximal promoters with TAF4b-precipitated chromatin relative to IgG. (B) The <i>Nobox</i> proximal promoter, in particular, demonstrates enrichment for TAF4b, in contrast to a non-genic region upstream of the promoter. For all analyses, data from each primer set were normalized to the E18.5 mouse ovary genomic DNA input levels and represented as a percentage of that DNA input. Each qPCR reaction was performed in triplicate and averaged. Error bars indicate the normalized standard deviation resulting from experimental triplicate qPCR reactions.</p
