<p>Phytochemical investigation of the <i>n</i>-hexane fraction of the methanolic extract of <i>Tagetes minuta</i> L. (Asteraceae) aerial parts afforded a new thiophene derivative: thiotagetin A (<b>3</b>), together with β-sitosterol (<b>1</b>) and stigmasterol (<b>2</b>). The structure of the new thiophene was identified by UV, IR, 1D (<sup>1</sup>H and <sup>13</sup>C), 2D (<sup>1</sup>H–<sup>1</sup>H COSY, HSQC and HMBC) NMR and HRESIMS spectral data. Compound <b>3</b> displayed cytotoxic activity against KB and MCF7 cancer cell lines with ED<sub>50</sub> values of 2.03 and 3.88 μg/mL, respectively, compared to adriamycin (0.26 and 0.07 μg/mL, respectively).</p
