Second strand synthesis yields.

Abstract

<p>(A) Box plots of second strand syntheses yields by starting amount of ssDNA indicate scalable and reliable yields. (B) Box plots comparing yields of lesion-free versus lesion-containing (8-oxoG, 5-OHU, or DHU) constructs, per 40 μg of ssDNA, or as expected per 40 μg of ssDNA if actual starting ssDNA amount was less (20–30 μg). Similar yields are obtained for lesion-free and lesion-containing constructs. Whiskers extend 1.5 times the interquartile range from the 25<sup>th</sup> and 75<sup>th</sup> percentiles, which are indicated by box limits. Center lines indicate the medians and circles represent the values for individual preparations. Determined by R software.</p

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