原著和名: ウメモドキ科名: モチノキ科 = Aquifoliaceae採集地: 愛知県 豊橋市 岩崎町 (三河 豊橋市 岩崎)採集日: 1968/10/20採集者: 萩庭丈壽整理番号: JH042317国立科学博物館整理番号: TNS-VS-99231

Alena Shkumatava (3577880)

Alexis Eschstruth (535535)

Hélène Eckert (3577886)

Jean-Baptiste Renaud (230969)

Jean-Paul Concordet (269984)

Jean-Stephane Joly (3577883)

Jim Kent (274755)

Joffrey Mianné (3407417)

Kai Schönig (261411)

Lydia Teboul (106309)

Maximilian Haeussler (86348)

Sylvie Schneider-Maunoury (2557627)

Ratio of off-target to on-target cleavage for validated guide sequences. The two guides RAG1B and RAG1A are not shown on this plot as their on-target cleavage was not determined in the study by Frock et al. [28]. Studies in the legend are referenced by the first author’s name; in the case of Kim et al., the cell type is also indicated. For each guide, the sum of all off-target modification frequencies was divided by the on-target modification frequency, e.g., a ratio of 2 indicates that cleavage is twice as frequent on all off-targets taken together than on the target. To better show the two outliers, a portion of the x-axis and y-axis was cut out. The CRISPOR website and Genome Browser tracks show a warning message for guide sequences with a GC content >75 %. (PDF 66 kb

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Additional file 6: Figure S2. of Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR

Precision/recall curves for the large-scale datasets. The precision/recall plot for the Wong score on the Chari dataset looks different to that in the Wong et al. article as our study is analyzing only full datasets and Wong et al. used cross-fold data. The datasets by Hart, Doench 2016, Koike-Yusa, or Wang/Xu give the best impression of the Wong score on independent data that was not used for training by Wong et al. (PDF 87 kb

Additional file 16: Figure S6. of Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR

Correlations between knock-out efficiency results in different studies and cell types. (XLS 40 kb

Additional file 11: Table S6. of Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR

MIT specificity scores calculated by the CRISPOR website for 28 guide sequences calculated based on predicted off-targets with up to four, five, and six mismatches. Only one label is shown for identical guide sequences from two different studies. A change from four to five allowed mismatches used in the scoring results in a change of the specificity scores but only in minor changes of the ranking of the guide sequences by specificity score. (PDF 23 kb

Additional file 7: Figure S3. of Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR

https://figshare.com/articles/Additional_file_16_Figure_S6_of_Evaluation_of_off-target_and_on-target_scoring_algorithms_and_integration_into_the_guide_RNA_selection_tool_CRISPOR/4466036

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