<p>(A-B) Represent unpigmented barbs from the control feather and 60°C feather respectively. (C- D) Brown barb and barbule from control feather. (C) Represents barb where cortex (arrow) and inner pith are visible. Note melanosomes (arrowhead) are sparse but present only in barb cortex. (D) Barbule with melanosomes (arrow) from control feather. (E) 60°C brown barb with barbule extending from the left side. Melanosomes are concentrated in the barbule and appear partially degraded as indicated by the less dense (arrowhead) and even ‘hollow’ (arrow) centers observed in the inset. (F) Pith from a rachis taken from the 60°C condition, embedded and sectioned separately (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0157699#sec005" target="_blank">Methods</a>). Note: Whether pith derives from the rachis (F) or barb (C) is impossible to determine at this level of magnification.</p
