<p>A) Cell Titer Glo (Promega) was used to assess the levels of ATP in 3D liver tissues over time. Data shown is the average of 3 replicates +/- standard deviation. B) Albumin was measured in the supernatant of 3D liver tissues over 28 days in culture by ELISA. Data shown is the average of 5 replicates +/- standard deviation. C) Levels of ATP in 3D liver tissues (A) were compared to those from standard 2D hepatocyte culture over 28 days by normalizing to day 3 levels. Data shown is the average of 3 replicates +/- standard deviation. D) Levels of albumin (ALB) in 3D liver tissues (B) were compared to those from standard 2D hepatocyte culture over 28 days by normalizing to day 3 levels. Data shown is the average of at least 5 replicates +/- standard deviation. All statistics (One way ANOVA) and outliers (Grubbs’ test) were calculated using GraphPad Prism software; * p<0.05, ** p<0.01, ***p<0.001, **** p<0.0001.</p
