<p>A) Measurement of secreted factor albumin in the supernatant of 3D liver tissues treated with Trovafloxacin or Levofloxacin daily for 7 days. Data is expressed as the percentage relative to the vehicle average and is the average of 10 replicates across two independent experiments +/-standard deviation. B) Measurement of ATP levels in 3D liver tissues treated with Trovafloxacin or Levofloxacin daily for 7 days. Data is expressed as the percentage relative to the vehicle average and is the average of 6 replicates across two independent experiments +/-standard deviation. C) Levels of albumin (ALB) in 3D liver tissues treated with Trovafloxacin (A) were compared to those from standard 2D hepatocyte cultures treated with Trovafloxacin daily for 7 days by normalizing to vehicle control. Data shown for 2D cultures is the average of 4 replicates +/- standard deviation. D) Levels of ATP in 3D liver tissues treated with Trovafloxacin (B) were compared to those from standard 2D hepatocyte cultures treated with Trovafloxacin daily for 7 days by normalizing to vehicle control. Data shown for 2D cultures is the average of 4 replicates +/- standard deviation. All statistics (One way ANOVA for 7A, B; Two way ANOVA for 7C, D) and outliers (Grubbs’ test) were calculated using GraphPad Prism software; * p<0.05, ** p<0.01, ***p<0.001, **** p<0.0001.</p
