<p>Treatment of L6 myotubes with Dex (100nM, 48hrs) decreased (A) total CRTC1 (p = 0.004 and CRTC2 protein (p = 0.0007). n = 9/treatment from 3 experiments. Representative western blots and corresponding sections of the Ponceau S-stained membranes are shown. Dex decreased (B) nuclear CRTC1 (p = 0.04) and nuclear CRTC2 (p = 0.03) protein and (C) nuclear CnA protein (p = 0.04); no differences in the cytosolic levels of these proteins were detected. Dex increased (D) nuclear SIK1 protein (p = 0.005) with no detectable difference in cytosolic protein. n = 3-7/treatment from 3–5 experiments. (E) Representative western blots for cytosolic and nuclear CRTC1, CRTC2, SIK, and CnA proteins are shown; controls for the nuclear (Histone) and cytosolic (GAPDH) fractions are also shown. (F) IBMX (250uM, 15 min) increased nuclear CRTC2 protein. A representative western blot image and corresponding Ponceau S-stained membrane from a single experiment run on the same gel are shown; the experiment was repeated one other time with similar results. In panels A-D, data are expressed as mean percent of control ± s.d and were analyzed by Student’s <i>t</i>-test. Asterisks indicate a value that is significantly different from control: p<0.05 = *, p<0.01 = ** and p<0.001 = ***.</p
