<p>(A) Cells with Hsp104 endogenously tagged with mCherry and ectopically expressing Guk1-GFP or Guk1-7-GFP were grown at 25°C and then incubated at 25°C or 37°C for 30 minutes before fixation and imaging. Scale bar represents 5μm. (B) Hsp42-mCherry cells ectopically expressing Guk1-GFP or Guk1-7-GFP were grown at 25°C prior to incubation at 25°C or 37°C for 30 minutes. Cells were then fixed before imaging. The scale bar represents 5μm. (C) Enlarged images from cells collected as in A. Scale bar represents 2.5μm. (D) Enlarged images from cells collected as in B. Scale bar represents 2.5μm.</p
