Changes in NvashA, Nvath-like, and NvsoxB2 expression following drug treatments or in with increased Notch activity. mRNA in situ hybridization for NvsoxB(2) (A) and NvashA (B) in DMSO-treated control animals grown to early planula stages (48 hpf at 22 °C). NvsoxB(2) (A’) and NvashA (B’) expression in same stage animal treated with U0126 from 24 to 48 hpf or treated with SU5402 from 24 to 48 hpf (B”). mRNA in situ expression of Nvath-like (C) and NvsoxB(2) (D) in control embryos injected with the venus mRNA. Expression of Nvath-like (C’) and NvsoxB(2) (D’) in animals injected with NvnotchICD:venus (the intracellular domain of the Notch receptor), which has been previously shown to hyperactivate Notch signaling. Embryos were classified and quantified as the percent having normal expression, weak expression, or no expression. The phenotypic class with the highest percentage of embryos is indicated. In C and D, the main figure panels are ectodermal focal planes, and insets show deeper focal planes used to confirm embryonic stage. All images are of lateral views with the oral side to the left. (TIF 34682 kb
