Changes in the proportions of intraocular macrophages and peripheral blood monocytes in splenectomized mice after laser injury.

Abstract

<p>(A) Less Ly6C^hi cells were recruited into the eye in splenectomized mice than those in controls both before laser treatment and at day 3 after laser injury, and intraocular Ly6C^int/loCD64⁺ cells were also reduced by splenectomy from before laser photocoagulation up to 7 days after laser, whereas, there were no significant changes in Ly6C^lo cells between two groups with or without laser injury. (B) In the peripheral blood, a lower proportion of Ly6C^int cells was observed before laser treatment, at day 3 and day 7 after laser injury, and Ly6C^lo cells were also reduced at day 7 after laser injury. In contrast, peripheral blood Ly6C^hi monocytes showed no differences between controls and splenectomized mice. All experiments were performed in triplicate. *P < 0.05 versus control of the same subtype with Dunn's multiple comparison test for post-hoc analysis.</p