Changes in the proportions of circulating monocyte/macrophage subtypes in peripheral blood after laser injury.

Abstract

<p>(A) Representative flow cytometry plots from WT mice. Monocyte subpopulations were gated based on Ly6C and CD43 expression to determine the proportions of classical (Ly6C^hiCD43^lo), intermediate (Ly6C^intCD43^hi) and nonclassical (Ly6C^loCD43^hi) monocytes per total leukocytes. Hi, int, and lo correspond to Ly6C^hi, Ly6C^int, and Ly6C^lo cells, respectively. (B) The proportion of circulating Ly6C^hi cells were significantly higher at day 3 after laser injury, whereas there were no changes in the proportions of Ly6C^lo and Ly6C^int cells. All experiments were performed in triplicate. *P < 0.05 versus control of the same subtype with Dunn's multiple comparison test for post-hoc analysis.</p