(A–C”) Autophagic flux reporter expression in ey > hid-p35 eye discs. Late third instar larval eye discs expressing the autophagic flux reporter GFP-mCherry-dAtg8a under control of the dAtg8 promoter [76]. The yellow dotted lines indicate the anterior portions of the eye discs which expresses ey-Gal4. Note the overgrowth of the anterior eye disc portion in ey > hid-p35 imaginal discs (B–B”). Expression of GFP and mCherry is low in the control ey > p35 discs (A–A”). In contrast, the numbers of GFP and mCherry positive particles are strongly increased in the overgrown ey-Gal4 expressing area of ey > hid-p35 discs (B–B”). Although the overgrowth of ey > hid-p35 eye discs is strongly suppressed by dAtg1 RNAi, the GFP and mCherry signals are not significantly reduced (C–C”). (TIF 7456 kb
