<p>A, B) Crushed sciatic nerve (2 weeks after lesion) was immunolabeled for CD8 T cells (green) and neurofilaments (axonal marker, red). Note CD8 T cells in the endoneural environment (B, detail). Scale bars: A, 50 μm; B, 20 μm. C-E) Crushed sciatic nerves were dissected at 2, 4 and 8 weeks after lesion (wal) and submitted to flow cytometry procedures. C) Representative graphs of CD8 T cell quantification (D) and cytokine expression (E). Note that the frequency of CD8 T cells diminishes over time (D), and these lymphocytes gradually express less INF-γ and more IL-10 (E). Data are presented as the mean ± SEM. n = 6 in each time point. **p<0.01; ***p<0.001 according to the one-way ANOVA followed by Bonferroni post-tests. F-H) H&E stained nerves at 2 (F), 4 (G) and 8 (H) weeks after lesion (wal), evidencing inflammation resolution. Scale bars: 50 μm.</p
