<p>A) FACS analysis of wild-type (UPY464), <i>mcm2DENQ</i> (UPY499), and <i>mrc1Δ</i> (UPY713). Briefly, strains were arrested in G1 by addition of α-factor and released into fresh YPD (T = 0). Aliquots were taken at the indicated times and processed for FACS as described in Materials and Methods. B) Cell death assay. Asynchronous cultures of indicated strains from A) ± 3 mM hydrogen peroxide (positive control) were assayed for cell death (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1006277#sec020" target="_blank">Materials and Methods</a>). Fluorescence (i.e., cell death) and phase contrast images are shown. C) Percent of dead cells observed in the indicated strains during asynchronous growth. Strains assayed as indicated in A) plus, <i>mrc1Δ rad9Δ sml1Δ</i> (UPY715).</p
