<p>A new 5(6→7)abeo-sterol with rarely reported [6–5-6–5]-fused rings, 3<i>β</i>,5<i>β</i>,6-trihydroxyl-<i>B</i>-norsitostane (<b>1</b>), along with 10 known compounds were isolated from the twigs of <i>Podocarpus fleuryi</i>. Their structures were elucidated by means of extensive spectroscopic analysis. Compound <b>1</b> was assessed for their cytotoxicity against five human tumour cell lines (HL-60, SMMC-7721, A-549, MCF-7 and SW-480), and the result showed that it had no activity.</p