Deletion of <i>Setd7</i> specifically in IECs renders mice resistant to <i>T</i>. <i>muris</i>.

Abstract

<p>(A) <i>Setd7</i><sup>f/f</sup> (black bars) and <i>Setd7</i><sup>ΔIEC</sup> (grey bars) mice were infected with 200 <i>T</i>. <i>muris</i> eggs and worm burdens were determined at day 14 or day 21 post infection (n≥9 for day 14, n = 6 for day 21, *** P<0.001). (B) Mesenteric lymph node cells from infected mice were re-stimulated for 72 h. IL-13 and IFN-γ concentrations in supernatants was determined by ELISA. (n≥4). (C) Expression of <i>Tslp</i> and <i>Muc2</i> in proximal colon at day 14 post infection with <i>T</i>. <i>muris</i> was assessed by qPCR. Expression is relative to infected control (<i>Setd7</i><sup>f/f</sup>) mice. (n≥8). (D) Protein levels of RELMβ that was secreted into the gut lumen of naïve and <i>T</i>. <i>muris</i> infected mice evaluated by Western blot.</p

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