<p>PBMC from three donors were activated and stimulated with refolded HLA-F, HLA-control tetramers, or no protein as described in <i>Materials and Methods</i>. (<b>A)</b> A representative FACS staining for the analysis of CD107a expression on cell populations gated in the top panel indicated as KIR3DS1+ and KIR3DS1-. Expression of CD107a was detected with percentages of the total cell population analyzed in bold in the lower right quadrant of each profile. Experiments were performed in the presence of HLA-F and D<sup>b</sup>-TRP bound to streptavidin beads and no-protein as indicated above the panels. The percentages CD107a positive cells within the KIR3DS1+ populations (<b>B</b>) and in the KIR3DS1- population (<b>C</b>) for each donor are graphed for comparison. <b>(B and C)</b> Five experiments were performed for each of four KIR3DS1+ donors (Donors CW and EB: KIR3DL1/KIR3DS1, Donors TG and DG KIR3DS1/KIR3DS1). The graphs show the percentage of total cells using the formula showing the mean of 5 replicates for each individual, the error bars represent the standard error of the mean (SEM) of the replicates, and the p-values calculated by performing a paired t-test comparing all values F and D<sup>b</sup> across replicates and individuals.</p
