Pericytes were treated for four consecutive days (once every 24 h) with either vehicle (Veh), TNFα (5 ng/mL), or IL-1β (1 ng/mL). After 48 h of cytokine treatment, cells were treated with either vehicle or PDGF-BB (10 ng/mL) to measure the PDGF-BB-induced proliferative response (A, B). This was done in two ways: after 96 h total treatment, cells were fixed, labelled with a Ki67 antibody and Hoechst (A, C); alternatively, EdU was added to measure cell proliferation over the final 24 h of the experiment (B, C). Positive cells of the total cells measured by Hoechst were quantified and plotted as mean ± s.e.m. (n = 3), ****(p < 0.0001), ***(p < 0.001), *(p < 0.05) from a two-way ANOVA. (TIF 1034 kb
