Pericytes were treated for four consecutive days (once every 24 h) with either vehicle (Veh), TNFα (5 ng/mL), or IL-1β (1 ng/mL). After 96 h total treatment, cells were serum starved for 2 h and then treated with vehicle (−) or PDGF-BB (100 ng/mL) for 30 min. PDGFRβ puncta were quantified using MetaXpress™ software and normalized to cell number and vehicle control and plotted as mean ± s.e.m. (n = 3), ***(p < 0.001), *(p < 0.05) (two-way ANOVA). Note: Control data are from the same experiments as Fig. 3g. (TIF 723 kb
