<p>(<b>A</b>) <b>a-e</b>, Live imaging of the mitochondrial morphology in the flight muscle of adult flies. The mitochondria are labeled with <i>UAS-mitoGFP</i> driven by <i>Mhc-Gal4</i> (<i>Mhc>mitoGFP</i>). The genotype is indicated on each micrograph. <b>f</b>, To quantify the mitochondrial size, the averaged mitochondrial size of the control (+/+) is set as 1, and the relative ratios of the other genotypes to the control are shown. Five thoraces from each genotype were quantified. Bar graphs throughout all figures are means ± SD. The white bar represents the control, the gray bar represents no statistical different from the control, and the black bar represents significantly different from the control. * for p<0.05; ** for p<0.01; ***for p<0.001. (<b>B</b>) Mitochondrial distribution and morphology in larval oenocytes. <b>a</b>, The mitochondria are labeled with <i>PromE(800)> mitoGFP</i>. <b>b-e</b>, The effects of <i>Khc</i>, <i>Milton</i>, <i>Miro</i> and <i>vimar RNAi</i> are shown. The dotted red lines denote the cell boundaries, which were determined by the mitoGFP background. <b>a'-e'</b>, Enlarged view of the white box labeled area in the upper panel. <b>f</b>, To quantify the mitochondrial length, the averaged mitochondrial length of the control (+/+) is set as 1, and the relative ratios of the other genotypes to the control are shown. Mitochondrial length of five oenocytes was quantified per genotype and shown as means ± SD. (<b>C</b>) Live imaging of mitochondria in eye disc after knocking down <i>vimar</i> by <i>GMR>mitoGFP</i>. Three eye discs were analyzed for each genotype. (<b>D</b>) Effect of <i>vimar</i> on mitochondrial transport. The mitochondria are labeled with mitoGFP (<i>CCAP>mitoGFP</i>), and their movements in the axons were recorded and transformed into kymographs. Mitochondria motion in ten axons from five larvae was analyzed for each genotype. The quantification is shown on the bar graph. (<b>E</b>) Subcellular vimar protein distribution by protein fractionation. The proteins from adult thoraces (<i>Mhc>MitoGFP</i>) were separated into cytosolic and crude mitochondrial fractions. The vimar protein enrichment was analyzed by immunobloting with the anti-vimar antibody. The mitoGFP protein was detected by the anti-GFP antibody; and β-actin is a cytosolic protein.</p
