<p>A. Transmission reducing activity of sera collected 2 weeks after each initial vaccination and 8 weeks after the booster vaccination. Each data point represents the average of two results in the SMFA, and lines indicate estimate EC50 and point-wise confidence interval by GEE model. B. Standard deviation (SD) of individual TRA from replica assays. Thick blue line indicates the threshold representing significant biological activity (50% TRA), the black line is a standard loess smooth curve. The green, red, blue, and black dots correspond to the data points on D70, D134, D314 (2 weeks after the second, third, and fourth vaccination respectively), and D356 (8 weeks after the fourth vaccination), as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0163144#pone.0163144.g004" target="_blank">Fig 4A</a>.</p
