Protein sequence alignment of Romo1/Mgr2, Tim17 and Tim23 from Saccharomyces cerevisiae, Allomyces macrogynus, Homo sapiens, Monodelphis domestica and Trichoplax adherens as performed by MAFFT [38]. The grey cylinders denote the transmembrane regions. The identical and similar residues were highlighted by turquoise and green color, respectively. The threshold value for shading was set to 50 %. (PDF 25 kb