Additional file 2: of Human cytomegalovirus reactivation from latency: validation of a “switch” model in vitro

Abstract

THP-1 macrophage (lytic model) infection with TB40E progeny derived from the THP-1 reactivation model. THP-1 macrophages (lytic model) were infected with the cell culture supernatant derived from the THP-1 reactivation model (as detailed in the Methods section), which had been infected with the TB40E strain at MOI of 0.5 (panels a, a’), 0.25 (panels b, b’) or 0.125 (panels c, c’) for 7 days; panels d, d’: uninfected cells. A–At 24 h p.i., THP-1 macrophages were fixed and labelled with an anti-IE antibody (“IE-positive THP-1 macrophages from the THP-1 reactivation model”). B–at 72 h p.i. they were fixed and labelled with an anti-pp65 antibody (“pp65-positive THP-1 macrophages from THP-1 reactivation model”). Bar: 25 μm. (PDF 1315 kb