20E increased the expression of osiris genes in the midgut. 20E injection was performed as described in methods. (A–B) Expression of osiris genes during the indicated developmental stages as assayed by qRT-PCR. Osiris genes assayed here were up-regulated at the early molting stage (6 h after the initiation of molting). (C–J) 20E injection induced the expression of osiris genes in the midgut. All osiris genes were up-regulated at 24 h post 20E injection. The changes were not obvious at other time points except Osiris 9-4 at 15 h post 20E injection. (PPT 330 kb